Development of a Multiplex RT-PCR Assay for Simultaneous Detection of Ten Major Viral Pathogens of Wheat

Triticum mosaic virus (TriMV) and High plains (HPV), identified recently, have been considered among the major viruses that affect wheat. Carried by same vector, wheat curl mite, both of these cause yellowing stunting plants which are very similar to many other attacking This makes it difficult detect in different lines, posing a problem yield. paper highlights addition two multiplex RT-PCR based method already detected presence barley cereal yellow dwarf (B/CYDVs), soil-borne (SBWMV), spindle streak (WSSMV), (WSMV). The uses specific sets primers target TriMV HPV at 560 bp 490 bp, respectively, distinct such as B/CYDVs -PAV, -MAV, -SGV, -RPV, -RMV, WSSMV, SBWMV, WSMV 295, 175, 237, 400, 365, 154, 219, 193 respectively. forward primer for each was fluorescently tagged higher throughput manner capillary electrophoresis. All ten may be viewed peaks an electropherogram from electrophoresis corresponding their product sizes base pairs. advancement protocol allows detection all single test, thus improving diagnostic capability with only slight increase cost.